However, the current 2 nd generation sequencing machines are not suited for routine clinical applications. Sample preparation is labour intensive and complex, run times span several days to weeks, and data-analysis is a specialised task that requires high-powered servers and bioinformatics expertise.
Moreover, the promise of low sequencing costs is only realised when sequencing runs are completely filled, which in case of diagnostic assays means running large numbers of samples in parallel.
As a result, current 2 nd generation sequencers are only suited for research purposes and cannot yet fill “the diagnostic sequencing gap”. The total analysis can be performed without manual intervention, without highly trained lab staff and only takes one day.